| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| HSP60 (D6F1) XP® Rabbit mAb | 12165 | 20 µl | 60 kDa | Rabbit IgG |
| HSP70 Antibody | 4872 | 20 µl | 72, 73 kDa | Rabbit |
| HSF1 Antibody | 4356 | 20 µl | 82 kDa | Rabbit |
| BiP (C50B12) Rabbit mAb | 3177 | 20 µl | 78 kDa | Rabbit IgG |
| HSP40 (C64B4) Rabbit mAb | 4871 | 20 µl | 40 kDa | Rabbit |
| HSP90 (C45G5) Rabbit mAb | 4877 | 20 µl | 90 kDa | Rabbit IgG |
| Calnexin (C5C9) Rabbit mAb | 2679 | 20 µl | 90 kDa | Rabbit IgG |
| PDI (C81H6) Rabbit mAb | 3501 | 20 µl | 57 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The HSP/Chaperone Sampler Kit provides an economical means to investigate protein folding within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments with each antibody.
Storage
Background
HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). HSP70 and HSP90 interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP-dependent manner (1). HSP40 family proteins bind unfolded proteins and prevent their aggregation, and deliver unfolded protiens to HSP70 (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). HSP60 is also present in the cytosol of many cells and is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (4-7). Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones including calnexin, BiP and protein disulfide isomerase (PDI). Calenxin is a calcium-binding protein embedded in the ER membrane that retains newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (8,9). When protein folding is disturbed inside the ER, Bip synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists them to refold properly (10). PDI catalyzes the formation and isomerization of disulfide bonds required to reach a proteins native state (11). Heat shock gene transcription is regulated by a familly of heat shock factors (HSFs), transcriptional activators that bind to heat shock response elements (HSEs) located upstream of all heat shock genes (12). During attenuation from the heat shock response, HSF1 is repressed by direct binding of HSP70, HSP40/Hdj-1 and HSF binding protein 1 (HSBP1) (13).
- Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809-2816.
- Fan, C.Y. et al. (2003) Cell Stress Chaperones 8, 309-316.
- Jindal, S. et al. (1989) Mol Cell Biol 9, 2279-83.
- Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
- Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
- Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
- Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.
- Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19, 124-128.
- Williams, D.B. (2006) J. Cell Sci. 119, 615-623.
- Kohno, K. et al. (1993) Mol. Cell. Biol. 13, 877-890.
- Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32.
- Morimoto, R.I. (1998) Genes Dev. 12, 3788-3796.
- Satyal, S.H. et al. (1998) Genes Dev. 12, 1962-1974.
Background References
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.