Revision 1

#99891Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

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3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
ATM (D2E2) Rabbit mAb 2873 20 µl 350 kDa Rabbit IgG
Phospho-ATM (Ser1981) (D25E5) Rabbit mAb 13050 20 µl 350 kDa Rabbit IgG
Rad51 (D4B10) Rabbit mAb 8875 20 µl 37 kDa Rabbit IgG
BRCA1 (A8X9F) Rabbit mAb 14823 20 µl 220 kDa Rabbit IgG
BRCA2 (D9S6V) Rabbit mAb 10741 20 µl 380 kDa Rabbit IgG
Rad54 (D4W3Z) Rabbit mAb 15016 20 µl 84 kDa Rabbit IgG
p95/NBS1 (D6J5I) Rabbit mAb 14956 20 µl 95 kDa Rabbit IgG
Phospho-p95/NBS1 (Ser343) Antibody 3001 20 µl 95 kDa Rabbit 
CtIP (D76F7) Rabbit mAb 9201 20 µl 110 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Homologous Recombination (HR) DNA Repair Antibody Sampler Kit provides an economical means of detecting proteins involved in HR DNA repair. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells recognize and repair DSBs via two distinct but partly overlapping signaling pathways, non-homologous end joining (NHEJ) and homologous recombination (HR). DSBs that arise during S or G2 phase are repaired via HR, using the replicated sister chromatid as a repair template (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA DSBs. ATM regulates various responses to DNA damage, including regulation of HR factors (2). Rad51 recombinase polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 (3). BRCA2 has been shown to be required for localization of Rad51 to sites of DSBs, and cells lacking BRCA1 and BRCA2 cannot repair DSBs through HR (4). NBS1 is critical for HR, and requires CDK-dependent association with CtIP and subsequent phosphorylation by ATM at Ser278 and Ser343 (5-6).

  1. Hartlerode, A.J. and Scully, R. (2009) Biochem J 423, 157-68.
  2. Lee, J.H. and Paull, T.T. (2007) Oncogene 26, 7741-8.
  3. Sung, P. et al. (2003) J Biol Chem 278, 42729-32.
  4. Tutt, A. and Ashworth, A. (2002) Trends Mol Med 8, 571-6.
  5. Wang, H. et al. (2013) PLoS Genet 9, e1003277.
  6. Wen, J. et al. (2013) Oncogene 32, 4448-56.

Background References

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