NOTE: Prepare solutions with Milli-Q or equivalently purified water.
NOTE: Add 1 mM PMSF immediately prior to use.
NOTE: For immunoprecipitations with immobilized primary antibody: Add 20 μl of immobilized antibody bead slurry to 200 μl cell lysate. Incubate with gentle rocking overnight at 4°C. Proceed to washing step 3.
posted June 2005
revised January 2012