How To Perform Antigen Retrieval (AR) in Immunohistochemistry (IHC)

We'll show you why antigen retrieval (AR) is important for immunohistochemistry (IHC). Antigen retrieval is required before incubating your tissue sample with antibodies, and can influence the strength of staining obtained in IHC. AR may be accomplished by treating the sample with either heat or enzymes. In this video, we'll give you tips on how to set up Heat Induced Epitope Retrieval using a microwave.