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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total LIN28A protein.


Species predicted to react based on 100% sequence homology: Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro201 of human LIN28A protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated LIN28A (D1A1A) XP® Rabbit mAb #8641.


LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).


1.  Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.

2.  Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.

3.  Cho, W.C. (2007) Mol Cancer 6, 60.

4.  Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.

5.  West, J.A. et al. (2009) Nature 460, 909-13.

6.  Ong, K.K. et al. (2009) Nat Genet 41, 729-33.

7.  Yu, J. et al. (2007) Science 318, 1917-20.


Entrez-Gene Id 79727
Swiss-Prot Acc. Q9H9Z2


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

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LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)