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12075S 100 µl (50 tests) $299.00


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Rabbit IgG

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total Ki-67 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human Ki-67 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human and rat cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ki-67 (D3B5) Rabbit mAb #9129.

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).

1.  Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.

2.  Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.

3.  Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.

4.  Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

Entrez-Gene Id 4288
Swiss-Prot Acc. P46013

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 647 Conjugate)