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56 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Brachyury Antibody recognizes transfected levels of total Brachyury protein.

Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly234 of human Brachyury protein. Antibodies are purified by protein A and peptide affinity chromatography.

Brachyury protein, encoded by the T gene, is a transcription factor that is vital for the formation of posterior mesoderm and axial development during vertebrate embryogenesis (1). In the mouse, brachyury is necessary for mesodermal morphogenetic cell movements during gastrulation. Brachyury mutant mice die in utero and display deficient mesoderm formation including an abnormal notochord, missing posterior somites, and a reduced allantois (2). Human brachyury is expressed in the notochord, as well as in chordoma tumors that occur along the spine, making it a good marker for notochord and notochord-derived tumors (3,4). A common polymorphism in the human T gene has also been shown to be associated with development of the multifactorial neural tube defect, spina bifida (5,6).

1.  Edwards, Y.H. et al. (1996) Genome Res 6, 226-33.

2.  Wilson, V. et al. (1995) Development 121, 877-86.

3.  Vujovic, S. et al. (2006) J Pathol 209, 157-65.

4.  Yang, X.R. et al. (2009) Nat Genet 41, 1176-8.

5.  Morrison, K. et al. (1996) Hum Mol Genet 5, 669-74.

6.  Jensen, L.E. et al. (2004) Hum Genet 115, 475-82.

Entrez-Gene Id 6862
Swiss-Prot Acc. O15178

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Brachyury Antibody