Upstream / Downstream
Explore pathways related to this product.
Important Ordering DetailsCustom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at firstname.lastname@example.org.
Research More. Spend Less.
Spend $650 or more and get 20% off*
(*Offer valid in the US only. Expires June 30, 2017)
Find answers on our FAQs page.
- Additional protein information
- Analytical tools
SignalKine™ Human IL-17A Chemiluminescent Sandwich ELISA Kit #12330
This product is discontinued
|Product Includes||Volume||Solution Color|
|hIL-17A Rabbit mAb Coated Microwells||96 tests|
|Human IL-17A Standard (Lyophilized)||1 vial|
|SignalKine™ Assay Diluent A01||1.5 ml||Colorless|
|SignalKine™ Sample Diluent S04||25 ml||Brown|
|hIL-17A Detection Rabbit mAb (Biotinylated) (Lyophilized) 100X||1 vial|
|Detection Antibody Diluent||6 ml||Green|
|HRP-linked Streptavidin (Lyophilized) 100X||1 vial|
|HRP Diluent||6 ml||Red|
|Luminol/Enhancer Solution||3 ml||Colorless|
|Stable Peroxide Buffer||3 ml||Colorless|
|Sealing Tape||2 sheets|
SignalKine™ Human IL-17A Chemiluminescent Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human IL-17A (hIL-17A) in multiple matrices. Unknown samples being tested for hIL-17A and hIL-17A standards are added to low volume microwells, where the hIL-17A is captured by the coated hIL-17A Rabbit mAb. Following a washing step, a biotinylated hIL-17A Detection Rabbit mAb is added to detect the captured hIL-17A. HRP-linked Streptavidin is then used for detection of the biotinylated hIL-17A Detection Rabbit mAb. A chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU) is proportional to the quantity of human hIL-17A in the sample.
SignalKine™ Human IL-17A Chemiluminescent Sandwich ELISA Kit detects hIL-17A in multiple matrices that can be quantified by generating a standard curve with the recombinant hIL-17A protein standard provided. The hIL-17A standard range is from 2 to 1500 pg/ml. Samples containing higher levels of hIL-17A can be diluted to fit into the standard range.
Matrices Tested: This ELISA has been validated in cell culture supernatants, plasma (citrate), plasma (EDTA), plasma (heparin), and serum.
IL-17A is a cystine-linked homodimeric pro-inflammatory cytokine produced by Th17 cells, a distinct CD4+ T cell lineage (1,2). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNF-α, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6, thereby bridging adaptive and innate immunity (1,2). IL-17A is intimately involved in mucosal immunity against bacterial infections (1,3) and has a putative role in some autoimmune disorders (1,4). IL-17A effects appear to be exerted primarily through binding to the IL-17RA (5). IL-17A binding induces production of cytokines, chemokines, and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (3,4,6).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.