The Plexin Isoform Antibody Sampler Kit provides an economical means of evaluating Plexin A1, A2, A3 and A4 protein expression. The kit contains enough primary antibody to perform four western miniblot experiments per primary antibody.
Plexin A1 Antibody detects endogenous levels of total Plexin A1 protein. Plexin A2 (D42B5) Rabbit mAb detects endogenous levels of total Plexin A2 protein and may cross-react with unidentified proteins at various molecular weights. Plexin A3 (D2G12) Rabbit mAb detects endogenous levels of total Plexin A3 protein and may cross-react with other family members. Plexin A4 (C5D1) Rabbit mAb detects endogenous levels of total Plexin A4 protein.
Polyclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Gln1875 of human Plexin A1 protein and purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly185 of human Plexin A2 protein, or Val440 of human Plexin A3 protein, or with a GST-fusion protein corresponding to residues from the human sequence of Plexin A4 protein.
Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3). Sema6A is a ligand for Plexin A2. Both Sema6A and Plexin A2 knock-out mice have a granule cell migration defect, where cells remain in the molecular layer. Furthermore, Plexin A2 also controls nucleus-centrosome coupling that modulates cell migration (4). Both Plexin A3 and A4 mediate the responses to class 3 semaphorins in sensory and sympathetic neurons. In particular, Plexin A4 is responsible for signaling of Sema 3A via neuropilin-1, while Plexin A3 is responsible for signaling of Sema 3F via neuropilin-2 (5).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Explore pathways related to this product.