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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CBFβ Antibody recognizes endogenous levels of total CBFβ protein.

Species predicted to react based on 100% sequence homology: Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro36 of human CBFβ protein. Antibodies are purified by protein A and peptide affinity chromatography.

Core-binding factor subunit β (CBFβ) is a non-DNA binding transcription factor subunit that associates with and regulates the DNA binding activity of RUNX1, RUNX2, and RUNX3 (1). CBFβ is ubiquitously expressed and has been implicated in a variety of developmental processes including hematapoiesis, T cell development, chondrogenesis, and bone formation (2-7). In addition, investigators have identified CBFβ as one of the most frequently translocated genes in leukemia (8) and research studies have found it to be required for HIV immune evasion (9,10). CBFβ interacts with the viral protein VIF and triggers assembly of a ubiquitin ligase complex that targets the retroviral inhibitor APOBEC3G for degradation (9,10).

1.  Ogawa, E. et al. (1993) Virology 194, 314-31.

2.  Wang, Q. et al. (1996) Cell 87, 697-708.

3.  Talebian, L. et al. (2007) Blood 109, 11-21.

4.  Johnson, K. et al. (2012) Science 336, 717-21.

5.  Yoshida, C.A. et al. (2002) Nat Genet 32, 633-8.

6.  Miller, J. et al. (2002) Nat Genet 32, 645-9.

7.  Kundu, M. et al. (2002) Nat Genet 32, 639-44.

8.  Goyama, S. and Mulloy, J.C. (2011) Int J Hematol 94, 126-33.

9.  Jäger, S. et al. (2012) Nature 481, 371-5.

10.  Zhang, W. et al. (2012) Nature 481, 376-9.

Entrez-Gene Id 865
Swiss-Prot Acc. Q13951

For Research Use Only. Not For Use In Diagnostic Procedures.
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CBFβ Antibody