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12946
PathScan® Phospho-M-CSF Receptor (Tyr699) Sandwich ELISA Kit

PathScan® Phospho-M-CSF Receptor (Tyr699) Sandwich ELISA Kit #12946

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Product Includes Volume Solution Color
M-CSFR Mouse mAb Coated Microwells 96 tests
Phospho-M-CSFR (Tyr699) Rabbit Detection Ab 1 ea Green (Lyophilized)
Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated) 1 ea Red (Lyophilized)
Detection Antibody Diluent 11 ml Green
HRP Diluent 11 ml Red
TMB Substrate 7004 11 ml
STOP Solution 7002 11 ml
Sealing Tape 2 ea
ELISA Wash Buffer (20X) 25 ml
ELISA Sample Diluent 25 ml Blue
Cell Lysis Buffer (10X) 9803 15 ml

The PathScan® Phospho-M-CSF Receptor (Tyr699) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of M-CSF receptor protein phosphorylated at Tyr699. A M-CSF receptor mouse mAb has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-M-CSF receptor proteins are captured by the coated antibody. Following extensive washing, Phospho-M-CSF Receptor (Tyr699) Rabbit Detection mAb is added to detect the captured phospho-M-CSF receptor proteins. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of M-CSF receptor protein phosphorylated at Tyr699.

Antibodies in kit are custom formulations specific to kit.

PathScan® Phospho-M-CSF Receptor (Tyr699) Sandwich ELISA Kit detects endogenous levels of M-CSF receptor protein phosphorylated at Tyr699 in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8).

Phosphorylation of M-CSF receptor at Tyr699 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery, and was cited in another publication (10). Autophosphorylation at Tyr699 within the kinase insert (KI) domain appears to provide a binding site for the Grb2 adaptor protein (9).

  1. Stanley, E.R. et al. (1978) Nature 274, 168-70.
  2. Byrne, P.V. et al. (1981) J Cell Biol 91, 848-53.
  3. Bourette, R.P. and Rohrschneider, L.R. (2000) Growth Factors 17, 155-66.
  4. Novak, U. et al. (1996) Oncogene 13, 2607-13.
  5. Bourette, R.P. et al. (1997) EMBO J 16, 5880-93.
  6. Morley, G.M. et al. (1999) Oncogene 18, 3076-84.
  7. Toy, E.P. et al. (2001) Gynecol Oncol 80, 194-200.
  8. Maher, M.G. et al. (1998) Clin Cancer Res 4, 1851-6.
  9. Hamilton, J.A. (1997) J Leukoc Biol 62, 145-55.
  10. Downing, J.R. et al. (1991) Mol Cell Biol 11, 2489-95.
Entrez-Gene Id
1436
Swiss-Prot Acc.
P07333
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

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Important Ordering Details

Custom Ordering Details: When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.