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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

SNF2H Antibody recognizes endogenous levels of total SNF2H (SMARCA5) protein. This antibody does not cross-react with SMARCA1 protein.

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig, Guinea Pig, Horse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala65 of human SNF2H (SMARCA5) protein. Antibodies are purified by protein A and peptide affinity chromatography.

Sucrose nonfermenting 2 homolog (SNF2H, SMARCA5) is one of two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). SNF2H is part of the SNF2 family of chromatin remodeling factors that use ATP hydrolysis to catalyze biochemical reactions in several mammalian chromatin-remodeling complexes, including ACF1, RSF1, CHRAC, NoRC, WSTF, and WCRF180 (2). Research studies show that SNF2H is crucial for chromatin organization, DNA damage response, and differentiation (1-7). The SNF2H helicase facilitates DNA damage repair by actively moving nucleosomes for DNA damage response (DDR) proteins to effectively associate with damaged regions (3). Additional studies show that repair of double stranded breaks (DSBs) significantly decreases in the absence of SNF2H (3), and these cells become highly sensitive to DNA damage caused by x-rays and chemical treatments inducing DSBs (4,5).

1.  Lazzaro, M.A. and Picketts, D.J. (2001) J Neurochem 77, 1145-56.

2.  Kasten, M.M. et al. (2011) Cell 144, 310.e1.

3.  Helfricht, A. et al. (2013) Cell Cycle 12, 3070-82.

4.  Mueller, A.C. et al. (2013) Oncogene 32, 1164-72.

5.  Lan, L. et al. (2010) Mol Cell 40, 976-87.

6.  Smeenk, G. et al. (2013) J Cell Sci 126, 889-903.

7.  Chioda, M. et al. (2010) Development 137, 3513-22.

Entrez-Gene Id 8467
Swiss-Prot Acc. O60264

For Research Use Only. Not For Use In Diagnostic Procedures.
CST is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.

SNF2H Antibody