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We recommend the following alternatives

W IP     F ChIP H
W IP     F ChIP H M XP
58,63-70,85 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Helios Antibody recognizes endogenous levels of total Helios protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro424 of human Helios protein. Antibodies are purified by protein A and peptide affinity chromatography.

Helios (Ikaros family zinc finger 2, IZKF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios gene IZKF2 is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4).

1.  Hahm, K. et al. (1998) Genes Dev 12, 782-96.

2.  Kelley, C.M. et al. (1998) Curr Biol 8, 508-15.

3.  Baine, I. et al. (2013) J Immunol 190, 1008-16.

4.  Holmfeldt, L. et al. (2013) Nat Genet 45, 242-52.

Entrez-Gene Id 22807
Swiss-Prot Acc. Q9UKS7

For Research Use Only. Not For Use In Diagnostic Procedures.
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Helios Antibody