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18653
SignalStain® Boost IHC Detection Reagent (AP, Rabbit)
Secondary Antibodies

SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653

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  1. IHC

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD8α (D8A8Y) Rabbit mAb #85336. Note: Detection was performed with SignalStain® Boost IHC Detection Reagent (AP, Rabbit) and SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713.

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Vimentin (D21H3) XP® Rabbit mAb #5741. Note: Detection was performed with SignalStain® Boost IHC Detection Reagent (AP, Rabbit) and SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713.

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma (left) and gastric adenocarcinoma (right) using Vimentin (D21H3) XP® Rabbit mAb #5741 (red) and E-Cadherin (4A2) Mouse mAb #14472 (brown).

SignalStain® Boost IHC Detection Reagent (AP, Rabbit) and SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713 were used to detect Vimentin (D21H3) XP® Rabbit mAb #5741. SignalStain® Boost IHC Detection Reagent (AP, Mouse) #31926 and SignalStain® DAB Substrate Kit #8059 were used to detect E-Cadherin (4A2) Mouse mAb #14472.

To Purchase # 18653S
Product # Size Price
18653S
15 ml $ 295

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Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa)
Isotype Horse 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

SignalStain® Boost IHC Detection Reagent (AP, Rabbit) is a ready-to-use solution intended for use in immunohistochemical assays to detect rabbit primary antibodies. SignalStain® Boost IHC Detection Reagent (AP, Rabbit) is a highly sensitive, one-step, polymer-based detection reagent that is specific for rabbit IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all alkaline phosphatase-based substrates.

Product Usage Information

1. Equilibrate SignalStain® Boost IHC Detection Reagent (AP, Rabbit) to room temperature prior to applying to the slides.

2. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature.

3. Wash slides and develop with any alkaline phosphatase-compatible substrate.

Storage:

SignalStain® Boost IHC Detection Reagent (AP, Rabbit) should be stored at 4°C and is stable for 12 months.

Specificity / Sensitivity

SignalStain® Boost IHC Detection Reagent (AP, Rabbit) can be used to detect rabbit primary antibodies in immunohistochemical assays.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.