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17 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cleaved IL-1beta (Asp116) Antibody detects endogenous levels of the 17 kDa processed form of IL-1beta. The antibody does not recognize the uncleaved precursor of IL-1beta.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of the 17 kDa mature form of human IL-1beta. Antibodies are purified by protein A and peptide affinity chromatography.

Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.

1.  Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99.

2.  Burns, K. et al. (1998) J Biol Chem 273, 12203-9.

3.  Cao, Z. et al. (1996) Nature 383, 443-6.

4.  Cao, Z. et al. (1996) Science 271, 1128-31.

5.  Wesche, H. et al. (1997) Immunity 7, 837-47.

6.  Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6.

7.  Thornberry, N.A. et al. (1992) Nature 356, 768-74.

8.  Cerretti, D.P. et al. (1992) Science 256, 97-100.

9.  Grahames, C.B. et al. (1999) Br J Pharmacol 127, 1915-21.

Entrez-Gene Id 3553
Swiss-Prot Acc. P01584

For Research Use Only. Not For Use In Diagnostic Procedures.
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Cleaved IL-1β (Asp116) Antibody