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2906
CDK4 (DCS156) Mouse mAb

CDK4 (DCS156) Mouse mAb #2906

This product is discontinued

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H Mk
Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

CDK4 (DCS156) Mouse mAb detects endogenous levels of total CDK4 protein. The antibody does not cross-react with other CDKs.

Monoclonal antibody is produced by immunizing animals with recombinant human CDK4 corresponding to amino acids 270-290.

Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle (4).

  1. Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
  2. Sherr, C.J. (1996) Science 274, 1672-7.
  3. Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
  4. Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9.
Entrez-Gene Id
1019
Swiss-Prot Acc.
P11802
For Research Use Only. Not For Use In Diagnostic Procedures.

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