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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cyclin D1 (DCS6) Mouse mAb detects endogenous levels of total cyclin D1 protein. The antibody does not cross-react with cyclin D2 or D3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human cyclin D1 corresponding to residues 151-170.

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).

Of the three D cyclins, aberrant expression of cyclin D1 has been associated with many forms of cancer including B cell lymphomas and can directly contribute to oncogenesis by various mechanisms including gene translocation or amplification (2). Cyclin D1 also plays a critical role in mammary tissue maturation (5).

1.  Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.

2.  Sherr, C.J. (1996) Science 274, 1672-7.

3.  Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.

4.  Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.

5.  Sicinski, P. et al. (1995) Cell 82, 621-630.

Entrez-Gene Id 595
Swiss-Prot Acc. P24385

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cyclin D1 (DCS6) Mouse mAb