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TH1L Antibody #3088
This product is discontinued
Gallery: TH1L Antibody #3088
TH1L Antibody recognizes endogenous levels of total TH1L protein.Species predicted to react based on 100% sequence homology: Hamster, Chicken, Xenopus, Bovine, Dog, Pig, Guinea Pig, Horse
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala132 of human TH1L protein. Antibodies are purified by protein A and peptide affinity chromatography.
Negative Elongation Factor (NELF) consists of four subunits: WHSC2 (NELF-A), COBRA-1 (NELF-B), TH1L (NELF-C/D), and NELF-E (1). NELF, together with DRB-sensitivity inducing factor (DSIF), inhibits RNA Polymerase II (RNAPII) elongation resulting in RNAPII promoter proximal pausing, where it waits additional signaling to resume transcription (2,3). The release of RNAPII from promoter proximal pausing is a critical regulatory point during transcription and is signaled by positive transcription elongation factor (p-TEF-b) phosphorylation of both NELF and the carboxy-terminal domain (CTD) within the largest subunit of RNAPII (3,4). WHSC2 is thought to connect the NELF complex to RNAPII machinery, while NELF-E contains an RNA binding motif that is necessary for NELF function (1,5,6). TH1L, together with COBRA-1, are integral subunits that bring WHSC2 and NELF-E together in the NELF complex (1).
In addition to its role in transcription regulation, TH1L is a negative regulator of MAPK signaling by inhibiting A-Raf and PAK1 kinase activities. Research studies have shown that TH1L expression is negatively correlated with breast cancer proliferation and migration (7-9).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc.