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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

TH1L Antibody recognizes endogenous levels of total TH1L protein.

Species predicted to react based on 100% sequence homology: Hamster, Chicken, Xenopus, Bovine, Dog, Pig, Guinea Pig, Horse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala132 of human TH1L protein. Antibodies are purified by protein A and peptide affinity chromatography.

Negative Elongation Factor (NELF) consists of four subunits: WHSC2 (NELF-A), COBRA-1 (NELF-B), TH1L (NELF-C/D), and NELF-E (1). NELF, together with DRB-sensitivity inducing factor (DSIF), inhibits RNA Polymerase II (RNAPII) elongation resulting in RNAPII promoter proximal pausing, where it waits additional signaling to resume transcription (2,3). The release of RNAPII from promoter proximal pausing is a critical regulatory point during transcription and is signaled by positive transcription elongation factor (p-TEF-b) phosphorylation of both NELF and the carboxy-terminal domain (CTD) within the largest subunit of RNAPII (3,4). WHSC2 is thought to connect the NELF complex to RNAPII machinery, while NELF-E contains an RNA binding motif that is necessary for NELF function (1,5,6). TH1L, together with COBRA-1, are integral subunits that bring WHSC2 and NELF-E together in the NELF complex (1).

In addition to its role in transcription regulation, TH1L is a negative regulator of MAPK signaling by inhibiting A-Raf and PAK1 kinase activities. Research studies have shown that TH1L expression is negatively correlated with breast cancer proliferation and migration (7-9).

1.  Narita, T. et al. (2003) Mol Cell Biol 23, 1863-73.

2.  Nechaev, S. and Adelman, K. (2011) Biochim Biophys Acta 1809, 34-45.

3.  Yamaguchi, Y. et al. (1999) Cell 97, 41-51.

4.  Buratowski, S. (2009) Mol Cell 36, 541-6.

5.  Yamaguchi, Y. et al. (2001) Science 293, 124-7.

6.  Yamaguchi, Y. et al. (2002) Mol Cell Biol 22, 2918-27.

7.  Cheng, C. et al. (2009) J Biol Chem 284, 8786-96.

8.  Liu, W. et al. (2004) J Biol Chem 279, 10167-75.

9.  Zou, W. et al. (2010) Cancer Sci 101, 2156-62.

Entrez-Gene Id 51497
Swiss-Prot Acc. Q8IXH7

For Research Use Only. Not For Use In Diagnostic Procedures.
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TH1L Antibody