Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Dec 31st Santa Cruz will discontinue a
large number of polyclonal products as
a result of the USDA settlement that was
made public May 19th.

Find CST Equivalent

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

We recommend the following alternatives

W IP         H
REACTIVITY SENSITIVITY MW (kDa) SOURCE
54 (Transfected only) Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-SGK (Ser78) Antibody detects transfected levels of SGK1 only when phosphorylated at serine 78. It will not detect isoforms SGK2 or SGK3.


Species predicted to react based on 100% sequence homology: Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser78 of human SGK. Antibodies are purified by protein A and peptide affinity chromatography.

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).


The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).


1.  Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.

2.  Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.

3.  Park, J. et al. (1999) EMBO J 18, 3024-33.

4.  Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.

5.  Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.

6.  Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.

7.  Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.


Entrez-Gene Id 6446
Swiss-Prot Acc. O00141


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

3271
Phospho-SGK (Ser78) Antibody