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19 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cofilin Antibody detects endogenous levels of total cofilin protein. The antibody may also recognize cofilin 2, the muscle isoform.

Species predicted to react based on 100% sequence homology: Pig

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser3 of human cofilin. Antibodies are purified by protein A.

Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

1.  Carlier, M.F. et al. (1999) J. Biol. Chem. 274, 33827-33830.

2.  Condeelis, J. (2001) Trends Cell Biol 11, 288-93.

3.  Arber, S. et al. (1998) Nature 393, 805-809.

4.  Yang, N. et al. (1998) Nature 393, 809-812.

5.  Toshima, J. et al. (2001) J. Biol. Chem. 276, 31449-31458.

6.  Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

Entrez-Gene Id 1072
Swiss-Prot Acc. P23528

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cofilin Antibody