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W IP         H Mk
40 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

RagB Antibody detects endogenous levels of total RagB protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RagB protein. Antibodies are purified by protein A and peptide affinity chromatography.

The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).

1.  Sancak, Y. et al. (2008) Science 320, 1496-501.

2.  Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.

3.  Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.

4.  Wullschleger, S. et al. (2006) Cell 124, 471-84.

Entrez-Gene Id 10325
Swiss-Prot Acc. Q5VZM2

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

RagB Antibody