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60, 80-90 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Myeloperoxidase (P733) Antibody detects endogenous levels of total myeloperoxidase. The antibody detects the heavy chain of myeloperoxidase.

Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro733 of human myeloperoxidase. Antibodies were purified by protein A and peptide affinity chromatography.

Myeloperoxidase (MPO) is a peroxidase enzyme that is part of the host defense system of polymorphonuclear leukocytes (reviewed in 1). The gene for MPO was cloned independently from several laboratories (2-5). A decrease in MPO expression was noticed upon differentiation of HL-60 cells (5). MPO catalyzes the reaction of hydrogen peroxide and chloride (or other halides) to produce hypochlorous acid and other potent antimicrobial oxidants. Knockout mice of MPO are impaired in clearing select microbial infections (6). Processing of mature MPO from an initial 80-90 kDa translation product involves insertion of a heme moiety, glycosylation, and proteolytic cleavage. The mature protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). It is abundantly expressed in neutrophils and monocytes and secreted during their activation. Heightened MPO levels have been associated with tissue damage and a number of pathological conditions (1).

1.  Klebanoff, S.J. (2005) J Leukoc Biol 77, 598-625.

2.  Chang, K.S. et al. (1986) Blood 68, 1411-4.

3.  Yamada, M. et al. (1987) Arch Biochem Biophys 255, 147-55.

4.  Morishita, K. et al. (1987) J Biol Chem 262, 3844-51.

5.  Weil, S.C. et al. (1987) Proc Natl Acad Sci USA 84, 2057-61.

6.  Aratani, Y. et al. (2002) J Infect Dis 185, 1833-7.

Entrez-Gene Id 4353
Swiss-Prot Acc. P05164

For Research Use Only. Not For Use In Diagnostic Procedures.
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Myeloperoxidase (P733) Antibody