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4278
Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb (Biotinylated)

Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb (Biotinylated) #4278

This product is discontinued

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H M R Mk XP
Storage:

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb (Biotinylated) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser240 and Ser244.

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser240 and Ser244 of human S6 ribosomal protein.

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb #4838 reacts with human, mouse, rat and monkey phospho-S6 ribosomal protein (Ser240/244). CST expects that Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb (Biotinylated) will also recognize phospho-S6 ribosomal protein (Ser240/244) in these species.

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

  1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
  2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
  3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
  4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
  5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.
Entrez-Gene Id
6194
Swiss-Prot Acc.
P62753
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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