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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Caspase-5 Antibody detects endogenous levels of total caspase-5 protein. This antibody detects the p20 subunit of active caspase-5.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu130 within the p20 subunit of human caspase-5 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Caspase-5 (Ich-3/ICErelIII/TY) is a member of the caspase family of cysteine proteases that play a key role in the execution of apoptosis and activation of inflammatory cytokines (1-3). Caspase-5 is widely expressed, with highest expression observed in placenta and lung (1). Interferon-γ and LPS regulate expression of caspase-5 (2,4). Members of the caspase-1 subfamily of caspases, which includes caspase-4, -5, and murine caspase-11 and -12, can induce apoptosis when over-expressed and mediate the proteolytic activation of inflammatory cytokines (5). Processing of IL-1β occurs through the activation of an inflammasome complex consisting of caspase-1, caspase-5, Pycard and NALP1 (6). Transcription factor Max, a component of the Myc/Mad/Max network, is cleaved by caspase-5 during Fas-induced apoptosis (7). Several alternative spliced variants of caspase-5 have been identified (8). Frameshift mutations of caspase-5 have been observed in leukemia, lymphoma (9), and colorectal cancers (10).

1.  Munday, N.A. et al. (1995) J Biol Chem 270, 15870-6.

2.  Wang, S. et al. (1996) J Biol Chem 271, 20580-7.

3.  Faucheu, C. et al. (1996) Eur J Biochem 236, 207-13.

4.  Lin, X.Y. et al. (2000) J Biol Chem 275, 39920-6.

5.  Martinon, F. and Tschopp, J. (2007) Cell Death Differ 14, 10-22.

6.  Martinon, F. et al. (2002) Mol Cell 10, 417-26.

7.  Krippner-Heidenreich, A. et al. (2001) Biochem J 358, 705-15.

8.  Eckhart, L. et al. (2006) Biochem Biophys Res Commun 348, 682-8.

9.  Takeuchi, S. et al. (2003) Leuk Res 27, 359-61.

10.  Trojan, J. et al. (2004) Int J Colorectal Dis 19, 538-44.

Entrez-Gene Id 838
Swiss-Prot Acc. P51878

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Caspase-5 Antibody