Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Stat5 (3H7) Rabbit mAb (Biotinylated) detects endogenous levels of total Stat5a and b protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a central region within human Stat5.
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions and tested in-house by western blot of human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (Stat5 (3H7) Rabbit mAb #9358).
Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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