Upstream / Downstream


Explore pathways related to this product.


Find answers on our FAQs page.


PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


16 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

p16 INK4A Antibody detects endogenous levels of p16 INK4A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within the carboxy-terminal region of human p16 INK4A. Antibodies are purified by protein A and peptide affinity chromatography.

Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins and phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase (1). The inhibitors of CDK4 (INK4) family include p15 INK4B, p16 INK4A, p18 INK4C and p19 INK4D. p18 has been shown to function as a haploinsufficient tumor suppressor in vivo (2). All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the amino-terminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6 (3). The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression (4,5).

p16 INK4A directly inhibits the activity of cyclin D, thereby inhibiting S-phase entry (6,7). As such, expression of p16 INK4A is commonly associated with cellular senescence, and disruption of the p16 INK4A gene is frequently observed in human cancers.

1.  Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.

2.  Bai, F. et al. (2003) Mol. Cell. Biol. 23, 1269-1277.

3.  Noh, S.J. et al. (1999) Cancer Res. 59, 558-564.

4.  Guan, K.L. et al. (1994) Genes Dev. 8, 2939-2952.

5.  Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.

6.  Sherr, C.J. (2001) Nat Rev Mol Cell Biol 2, 731-7.

7.  Lowe, S.W. and Sherr, C.J. (2003) Curr Opin Genet Dev 13, 77-83.

Entrez-Gene Id 1029
Swiss-Prot Acc. P42771

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

p16 INK4A Antibody