Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Phospho-FANCD2 (Ser222) Antibody detects endogenous levels of FANCD2 only when phosphorylated at Ser222.
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser222 of human FANCD2. Antibodies are purified by protein A and peptide affinity chromatography.
Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). In response to DNA damage, the FA nuclear complex (FANCA , FANCB, FANCC, FANCE, FANCF, FANCG, FANCM) induces mono-ubiquitination of FANCD2 and FANCI (2).
Monoubiquitination of FANCD2 induces localization of FANCD2 to sites of DNA damage, where it interacts with BRCA1 (4). FANCJ/BRIP1, FANCD1/BRCA2 and FANCN/PALB2 are also recruited to sites of DNA damage. FA signaling is important in maintenance of chromosome stability and control of mitosis (3).
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