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155 FANCD2-S. 230 FANCD2-L. Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-FANCD2 (Ser222) Antibody detects endogenous levels of FANCD2 only when phosphorylated at Ser222.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser222 of human FANCD2. Antibodies are purified by protein A and peptide affinity chromatography.

Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as MMC), and a predisposition to cancer (1). FANCD2 is the gene product of one of at least six genes thought to contribute heterogeneously to FA (2). MMC treatment results in ubiquitination of FANCD2 on Lys561, converting the protein from a short form (FANCD2-S) to a monoubiquitinated or long (FANCD2-L) form that localizes to subnuclear foci at sites of DNA damage with BRCA1, Rad51, and NBS1 (3-5). Ionizing radiation, on the other hand, results in ATM-dependent phosphorylation of FANCD2 at Ser222 and Ser1404 resulting in an S-phase arrest (4).

1.  Alter, B.P. (1996) Am J Hematol 53, 99-110.

2.  Timmers, C. et al. (2001) Mol. Cell 7, 241-248.

3.  Garcia-Higuera, I. et al. (2001) Mol. Cell 7, 249-262.

4.  Taniguchi, T. et al. (2002) Cell 109, 459-472.

5.  Nakanishi, K. et al. (2002) Nat. Cell Biol. 4, 913-920.

Entrez-Gene Id 2177
Swiss-Prot Acc. Q9BXW9

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Phospho-FANCD2 (Ser222) Antibody