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Phospho-IκBα (Ser32/36) (12C2) Mouse mAb #5210
Gallery: Phospho-IκBα (Ser32/36) (12C2) Mouse mAb #5210
Phospho-IκBα (Ser32/36) (12C2) Monoclonal Antibody can be used in high throughput kinase assays and drug discovery applications. It detects peptides derived from IκBα phosphorylated at Ser32/36.
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα. Antibody is purified by protein G chromatography.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. DELFIA is a registered trademark of PerkinElmer, Inc.