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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

OGT Antibody recognizes endogenous levels of total OGT protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala686 of human OGT protein. Antibodies are purified by protein A and peptide affinity chromatography.

O-GlcNAcylation is a post-translational modification where β-D-N-acetylglucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis, and transcription (2,3), and research studies have implicated this modification in cancer (1). The reversible protein modification by O-GlcNAc, which has been suggested to be a nutrient and stress sensor, is catalyzed by two highly conserved enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) (4).

1.  Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.

2.  Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.

3.  Capotosti, F. et al. (2011) Cell 144, 376-88.

4.  Hart, G.W. et al. (2007) Nature 446, 1017-22.

Entrez-Gene Id 8473
Swiss-Prot Acc. O15294

For Research Use Only. Not For Use In Diagnostic Procedures.
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OGT Antibody