Buy 3 Get a 4th Free* | Learn More >>
5382
Raptor (24C12) Rabbit mAb (Sepharose® Bead Conjugate)

Raptor (24C12) Rabbit mAb (Sepharose® Bead Conjugate) #5382

This product is discontinued

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Raptor (24C12) Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of total Raptor protein.

Raptor (24C12) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Raptor.

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. Raptor (24C12) Rabbit mAb (Sepharose® Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Raptor (24C12) Rabbit mAb #2280.

The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets (1,2). Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates (3,4). Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycin's specific inhibition of mTOR signaling (5). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (6). GβL is also part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR), and may mediate rictor-mTOR signaling to downstream targets including PKCα (7). Furthermore, the rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB on Ser473, facilitating phosphorylation of Akt/PKB on Thr308 by PDK1 and required for the full activation of Akt/PKB (8).

Recently raptor has been identified as a direct substrate of the AMP-activated protein kinase (AMPK) (9). AMPK phosphorylates raptor on Ser722/Ser792 (9). This phosphorylation is essential for inhibition of the raptor-containing mTOR complex 1 (mTORC1) and induces cell cycle arrest when cells are stressed for energy (9). These findings suggest that raptor is a critical switch that correlates cell cycle progression with energy status.

  1. Hara, K. et al. (2002) Cell 110, 177-189.
  2. Kim, D.H. et al. (2002) Cell 110, 163-175.
  3. Beugnet, A. et al. (2003) J. Biol. Chem. 278, 40717-40722.
  4. Nojima, H. et al. (2003) J. Biol. Chem. 278, 15461-15464.
  5. Oshiro, N. et al. (2004) Genes Cells 9, 359-366.
  6. Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.
  7. Sarbassov, D.D. et al. (2004) Curr Biol 14, 1296-302.
  8. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
  9. Gwinn, D.M. et al. (2008) Mol Cell 30, 214-26.
Entrez-Gene Id
57521
Swiss-Prot Acc.
Q8N122
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

Upstream / Downstream

pathwayImage

Explore pathways related to this product.