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30-42 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

NKX2.5 Antibody detects endogenous levels of total NKX2.5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human NKX2.5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

NKX2.5 is a member of the NKX homeobox transcription factor family. NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine Nkx2.5 gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2). Mutations in NKX2.5 are likewise associated with several congenital heart conditions, such as atrial defect with atrioventricular conduction defects (ASD-AVCD) and Tetralogy of Fallot (TOF) (3,4). Transcriptional activation of NKX2.5 is also associated with some B and T cell leukemias that result from chromosomal translocation (5-8).

1.  Lyons, I. et al. (1995) Genes Dev 9, 1654-66.

2.  Tanaka, M. et al. (1999) Development 126, 1269-80.

3.  Benson, D.W. et al. (1999) J Clin Invest 104, 1567-73.

4.  Reamon-Buettner, S.M. and Borlak, J. (2010) Hum Mutat 31, 1185-94.

5.  Nagel, S. et al. (2003) Cancer Res 63, 5329-34.

6.  Przybylski, G.K. et al. (2006) Haematologica 91, 317-21.

7.  Nagel, S. et al. (2007) Cancer Res 67, 1461-71.

8.  Su, X. et al. (2008) Haematologica 93, 1081-5.

Entrez-Gene Id 1482
Swiss-Prot Acc. P52952

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

NKX2.5 Antibody