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65 Rabbit 

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Occludin (D15G7) Rabbit mAb detects endogenous levels of total occludin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human occludin protein.

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1 (3). VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability (4). Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV) (5).

1.  Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.

2.  Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28.

3.  Rao, R. (2009) Ann N Y Acad Sci 1165, 62-8.

4.  Murakami, T. et al. (2009) J Biol Chem 284, 21036-46.

5.  Ploss, A. et al. (2009) Nature 457, 882-6.

Entrez-Gene Id 100506658
Swiss-Prot Acc. Q16625

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Occludin (D15G7) Rabbit mAb