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160 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-TBC1D1 (Thr590) Antibody recognizes transfected levels of TBC1D1 protein only when phosphorylated at Thr590.

Species predicted to react based on 100% sequence homology: Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr590 of mouse TBC1D1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified in skeletal muscle (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5).

1.  Taylor, E.B. et al. (2008) J Biol Chem 283, 9787-96.

2.  Roach, W.G. et al. (2007) Biochem J 403, 353-8.

3.  Stone, S. et al. (2006) Hum Mol Genet 15, 2709-20.

4.  Chavez, J.A. et al. (2008) J Biol Chem 283, 9187-95.

5.  Vichaiwong, K. et al. (2010) Biochem J 431, 311-20.

Entrez-Gene Id 57915
Swiss-Prot Acc. Q60949

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Phospho-TBC1D1 (Thr590) Antibody