Figure 1. Treatment of Raw264.7 cells with LPS stimulates expression of iNOS, detected by the PathScan® Total iNOS Sandwich ELISA Kit. Raw264.7 cells (80-90% confluent) were treated with 1 ng/ml LPS for 6 hours at 37º C. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using iNOS Antibody (Mouse Specific) #2982 is shown in the bottom figure.Learn more about how we get our images
|Product Includes||Volume||Solution Color|
|iNOS Ab Coated Microwells||1 ea|
|iNOS Detection Ab||11 ml||Green|
|Anti-mouse IgG, HRP-linked Antibody||11 ml||Red|
The PathScan® Total iNOS Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of iNOS. An iNOS rabbit antibody has been coated onto the microwells. After incubation with cell lysates, iNOS protein is captured by the coated antibody. Following extensive washing, an iNOS mouse detection antibody is added to detect the captured iNOS protein. Anti-mouse IgG, HRP-linked Antibody #7076 is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of iNOS.
Cell Signaling Technology's PathScan® Total iNOS Sandwich ELISA Kit #7097 detects endogenous levels of total iNOS protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. PathScan is a trademark of Cell Signaling Technology, Inc.
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