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PhosphoSitePlus® Resource

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REACTIVITY
Product Includes Volume Solution Color
FLT3 Mouse mAb Coated Microwells 96 tests
Phospho-FLT3 (Tyr591) Rabbit Detection Antibody 1 ea Green (Lyophilized)
Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated) 1 ea Red (Lyophilized)
Detection Antibody Diluent 11 ml Green
HRP Diluent 11 ml Red
STOP Solution 7002 11 ml Colorless
Sealing Tape 1 ea
ELISA Wash Buffer (20X) 25 ml Colorless
ELISA Sample Diluent 25 ml Blue
Cell Lysis Buffer (10X) 9803 15 ml Yellowish

Product Description

CST's PathScan® Phospho-FLT3 (Tyr591) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of FLT3 protein phosphorylated at Tyr591. A FLT3 Mouse mAb has been coated onto the microwells. After incubation with cell lysates, FLT3 (phospho or nonphospho) protein is captured by the coated antibody. Following extensive washing, a phospho-FLT3 (Tyr 591) Rabbit Antibody is added to detect the captured phospho-FLT3 protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of FLT3 phosphorylated at Tyr591.

Antibodies in kit are custom formulations specific to kit.


Specificity / Sensitivity

PathScan® Phospho-FLT3 (Tyr591) Sandwich ELISA Kit #7206 detects endogenous levels of FLT3 protein phosphorylated at Tyr591 in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).


1.  Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48.

2.  Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30.

3.  Namikawa, R. et al. (1996) Stem Cells 14, 388-95.

4.  Beslu, N. et al. (1996) J Biol Chem 271, 20075-81.

5.  Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9.

6.  Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80.

7.  Mizuki, M. et al. (2000) Blood 96, 3907-14.


Entrez-Gene Id 2322
Swiss-Prot Acc. P36888


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7206
PathScan® Phospho-FLT3 (Tyr591) Sandwich ELISA Kit