Fresh savings just popped up! 25% off 3 or more products. | Start Saving >>
7309
PathScan® Total β-Catenin Sandwich ELISA Antibody Pair

PathScan® Total β-Catenin Sandwich ELISA Antibody Pair #7309

This product is discontinued

 Image 1

The relationship between protein concentration of lysates from untreated and Forskolin treated COS cells and kit assay optical density readings is shown. COS cells were treated with Forskolin #3828 (50 µM) for 30 min at 37°C, and then lysed.

Learn more about how we get our images
Product Includes Volume Cap Color
Beta-Catenin Capture Ab (100X) 0.4 ml Pink
Beta-Catenin Detection Ab (100X) 0.4 ml Blue
Anti-mouse IgG, HRP-linked Antibody 0.04 ml Yellow

CST's PathScan® Total β-Catenin Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total β-Catenin Sandwich ELISA Kit #7308. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The β-Catenin Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a β-Catenin Detection Antibody and anti-Rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total β-Catenin protein.

Antibodies in kit are custom formulations specific to kit.

Storage:

Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.
Entrez-Gene Id
1499
Swiss-Prot Acc.
P35222
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.