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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cre Recombinase Antibody recognizes transfected levels of total Cre recombinase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp141 of Cre recombinase protein. Antibodies are purified by protein A and peptide affinity chromatography.

Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).

1.  Abremski, K. et al. (1983) Cell 32, 1301-11.

2.  Sternberg, N. et al. (1981) Cold Spring Harb Symp Quant Biol 45 Pt 1, 297-309.

3.  Abremski, K. and Hoess, R. (1984) J Biol Chem 259, 1509-14.

4.  Qin, M. et al. (1994) Proc Natl Acad Sci U S A 91, 1706-10.

5.  Lakso, M. et al. (1992) Proc Natl Acad Sci U S A 89, 6232-6.

6.  Guo, F. et al. (1997) Nature 389, 40-6.

Entrez-Gene Id 2777477
Swiss-Prot Acc. P06956

For Research Use Only. Not For Use In Diagnostic Procedures.
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Cre Recombinase Antibody