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Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

Dec 31st Santa Cruz will discontinue a
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a result of the USDA settlement that was
made public May 19th.

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Product Includes Volume Cap Color
Zap-70 Capture Ab (100X) 0.4 ml Pink
Zap-70 Detection Ab (100X) 0.4 ml Blue
Anti-rabbit IgG, HRP-linked Antibody (1000X) 0.04 ml Red

Product Description

CST's PathScan® Total Zap-70 Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Total Zap-70 Sandwich ELISA Kit #7172. Capture and detection antibodies (100X stocks) and HRP-linked secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Zap-70 capture antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Zap-70 detection antibody and anti-Rabbit IgG, HRP-linked antibody. HRP substrate,TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total Zap-70 protein.

Antibodies in kit are custom formulations specific to kit.


Product Usage Information

Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).


1.  Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.

2.  Iwashima, M. et al. (1994) Science 263, 1136-9.

3.  Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.

4.  Chan, A.C. et al. (1995) EMBO J 14, 2499-508.

5.  Williams BL et al. (1999) EMBO J 18, 1832–44

6.  Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.

7.  Wiestner, A. et al. (2003) Blood 101, 4944-51.

8.  Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.


Entrez-Gene Id 7535
Swiss-Prot Acc. P43403


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7850
PathScan® Total Zap-70 Sandwich ELISA Antibody Pair