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7852
PathScan® Phospho-Zap-70 (Tyr319) Sandwich ELISA Antibody Pair

PathScan® Phospho-Zap-70 (Tyr319) Sandwich ELISA Antibody Pair #7852

This product is discontinued

 Image 1

The relationship between protein concentration of lysates from untreated and H2O2-treated Jurkat cells and the absorbance at 450 nm using PathScan® Phospho- Zap-70 (Tyr317) Sandwich ELISA Antibody Pair #7852 is shown. Jurkat cells (0.8 x 106 cells/ml) were treated with H2O2 (2 mM) for 2 min at 25ºC and then lysed.

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Product Includes Volume Cap Color
Zap-70 Capture Ab (100X) 0.4 ml Pink
P-Zap-70 (Y319) Detection Ab (100X) 0.4 ml Blue
Anti-rabbit IgG, HRP-linked Antibody (1000X) 0.04 ml Red

CST's PathScan® Phospho-Zap-70 (Tyr317) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Zap-70 (Tyr317) Sandwich ELISA Kit #7171. Capture and detection antibodies (100X stocks) and HRP-linked secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Zap-70 capture antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Phospho-Zap-70 (Tyr317) detection antibody and anti-Rabbit IgG, HRP-linked antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho- Zap-70 (Tyr317) protein.

Storage:

Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

  1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
  2. Iwashima, M. et al. (1994) Science 263, 1136-9.
  3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
  4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
  5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
  6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
  7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
  8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.
Entrez-Gene Id
7535
Swiss-Prot Acc.
P43403
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.