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7921
SignalKine™ Human IL-8 Chemiluminescent Sandwich ELISA Kit

SignalKine™ Human IL-8 Chemiluminescent Sandwich ELISA Kit #7921

This product is discontinued

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Figure1. Supernatants from PBMCs, untreated or treated with PHA (10 µg/ml), were removed after 1 and 5 d. hIL-8 levels in the supernatants were quantified using SignalKine™ Human IL-8 Chemiluminescent Sandwich ELISA Kit.

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Typical Data. Represented here are hIL-8 Standard Curves, one diluted in SignalKine™ Sample Diluent S01, a second diluted in SignalKine™ Sample Diluent S03 and a third in cell culture media (RPMI + 10% FBS). Although these are typical of the standard curves that will be generated using this kit, a new set of standards should be run for each new experiment.

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Product Includes Volume Solution Color
hIL-8 Rabbit mAb Coated Microwells (Chemiluminescent) 96 tests
Human IL-8 Standard (Lyophilized) 1 vial
SignalKine™ Assay Diluent A01 1.5 ml Colorless
SignalKine™ Sample Diluent S01 25 ml Brown
SignalKine™ Sample Diluent S03 25 ml Brown
hIL-8 Detection Rabbit mAb (Biotinylated)(Lyophilized) 100X 1 vial
Detection Antibody Diluent 6 ml Green
HRP-linked Streptavidin (Lyophilized) 100X 1 vial
HRP Diluent 6 ml Red
ELISA Wash Buffer (20X) 25 ml Colorless
Luminol/Enhancer Solution 3 ml Colorless
Stable Peroxide Buffer 3 ml Colorless
Sealing Tape 2 ea

SignalKine™ Human IL-8 Chemiluminescent Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human IL-8 (hIL-8) in multiple matrices. Unknown samples being tested for hIL-8 and hIL-8 standards are added to low volume microwells, where the hIL-8 is captured by the coated hIL-8 Rabbit mAb. Following a washing step, a biotinylated hIL-8 Detection Rabbit mAb is added to detect the captured hIL-8. HRP-linked Streptavidin is then used for detection of the biotinylated hIL-8 Detection Rabbit mAb. A chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU) is proportional to the quantity of human hIL-8 in the sample.

SignalKine™ Human IL-8 Chemiluminescent Sandwich ELISA Kit detects hIL-8 in multiple matrices that can be quantified by generating a standard curve with the recombinant hIL-8 protein standard provided. The hIL-8 standard range is from 13.7 to 10,000 pg/ml. Samples containing higher levels of hIL-8 can be diluted to fit into the standards range.

Species Reactivity:

Human

IL-8 is the prototypical CXC chemokine. It is best known for effects on neutrophils, specifically its ability to act as a chemoattractant and activate degranulation and respiratory burst (1-3). IL-8 is produced by a number of cell types, including monocytes, T cells, neutrophils, fibroblasts, endothelial cells, and others (1). In addition to its effects on neutrophils, IL-8 promotes angiogenesis, inhibits endothelial cell apoptosis, and promotes the proliferation of melanoma cells in an autocrine fashion (1). As a result, IL-8 may be a contributing factor to the development of certain cancers (1,2). There are two distinct G protein-coupled receptors for IL-8, CXCR1 and CXCR2 (1). Ligand binding induces Ca2+ mobilization and activates the PI3K, PKC, Rho, and Rac pathways (1,3).

  1. Payne, A.S. and Cornelius, L.A. (2002) J Invest Dermatol 118, 915-22.
  2. Brat, D.J. et al. (2005) Neuro Oncol 7, 122-33.
  3. Mukaida, N. (2003) Am J Physiol Lung Cell Mol Physiol 284, L566-77.
Entrez-Gene Id
3576
Swiss-Prot Acc.
P10145
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.