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PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Antibody Pair

PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Antibody Pair #7993

This product is discontinued

Sensitivity Image 1

The relationship between the protein concentration of lysates from untreated and H202-treated Jurkat cells and the absorbance at 450 nm using the PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Antibody Pair is shown. Jurkat cells were treated with H202 (20 mM) for 3 minutes at 37ºC and then lysed.

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Product Includes Volume Cap Color
Phospho-Lck (Tyr505) Rabbit Capture Antibody (100X) 0.4 ml Pink
Lck Mouse Detection Antibody (100X) 0.4 ml Blue
Anti-mouse IgG, HRP-linked Antibody (1000X) 0.04 ml Yellow

Cell Signaling Technology's PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Kit #7941. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The phospho-Lck (Tyr505) rabbit capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysates are added followed by a total Lck mouse detection antibody and anti-mouse IgG, HRP-linked antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-Lck (Tyr505) protein.


Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Lck belongs to the Src-like non-receptor tyrosine kinase family with the typical Src family kinase structure: a unique amino terminal domain (Src homology 4 domain, SH4) followed by an SH3 domain, an SH2 domain, a kinase domain (SH1), and a carboxy-terminal negative regulatory domain (1). Lck activity is controlled by the interactions of SH2 and SH3 domains as well as tyrosine phosphorylation status of the activation loop (2,3). Lck is recruited to the T cell receptor (TCR) complex upon stimulation and activates downstream tyrosine kinases to initiate T cell signaling (4). Lck is also found to be involved in the regulation of mitochondrial apoptosis pathways and may be responsible for some anticancer drug induced apoptosis (5,6).

  1. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
  2. Mustelin, T. and Taskén, K. (2003) Biochem J 371, 15-27.
  3. Gervais, F.G. et al. (1993) Mol Cell Biol 13, 7112-21.
  4. Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93.
  5. Belka, C. et al. (2003) Oncogene 22, 176-85.
  6. Gruber, C. et al. (2004) Biochem Pharmacol 67, 1859-72.
Entrez-Gene Id
Swiss-Prot Acc.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

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Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at