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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

AIM2 Antibody recognizes endogenous levels of total AIM2 protein.

Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro250 of human AIM2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Absent in melanoma 2 (AIM2) is an interferon-inducible protein containing an amino-terminal pyrin domain and carboxy-terminal HIN-200 domain that functions in innate immunity and tumor progression (1). Expression of AIM2 can inhibit cell growth and tumor formation (2,3). Furthermore, the AIM2 gene has a high frequency of mutations associated with microsatellite-unstable colorectal cancers (4). AIM2 has a critical role in the activation of caspase-1, the protease responsible for the processing of pro-inflammatory cytokines IL-1β and IL-18. Caspase-1 activation is regulated by multi-protein complexes referred to as “inflammasomes” (5,6). Distinct inflammasome complexes have been described containing NLRP1/NALP1, NLRP3/NALP3, IPAF, and AIM2. The HIN-200 domain of AIM2 is responsible for binding to cytoplasmic double stranded DNA, resulting in caspase-1 activation. (7-9). This inflammasome complex also involves binding of the pyrin domain of AIM2 to the CARD-domain protein ASC/TMS1, which then interacts directly with caspase-1. As a result, AIM2 has been demonstrated to be an important sensor for a number of different pathogens (10-12).

1.  DeYoung, K.L. et al. (1997) Oncogene 15, 453-7.

2.  Chen, I.F. et al. (2006) Mol Cancer Ther 5, 1-7.

3.  Patsos, G. et al. (2010) Int J Cancer 126, 1838-49.

4.  Woerner, S.M. et al. (2007) Genes Chromosomes Cancer 46, 1080-9.

5.  Schroder, K. and Tschopp, J. (2010) Cell 140, 821-32.

6.  Khare, S. et al. (2010) Crit Rev Immunol 30, 463-87.

7.  Roberts, T.L. et al. (2009) Science 323, 1057-60.

8.  Hornung, V. et al. (2009) Nature 458, 514-8.

9.  Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13.

10.  Jones, J.W. et al. (2010) Proc Natl Acad Sci USA 107, 9771-6.

11.  Fernandes-Alnemri, T. et al. (2010) Nat Immunol 11, 385-93.

12.  Kim, S. et al. (2010) Eur J Immunol 40, 1545-51.

Entrez-Gene Id 9447
Swiss-Prot Acc. O14862

For Research Use Only. Not For Use In Diagnostic Procedures.
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AIM2 Antibody