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8199
Mouse Lipocalin-2 (mLCN2)

Mouse Lipocalin-2 (mLCN2) #8199

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Coomassie Gel Image 1

The purity of recombinant mLCN2 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mLCN2 and staining overnight with Coomassie Blue.

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Bioactivity Image 2

The viability of BAF3 cells treated with increasing amounts of mLCN2 was assessed. After 48 hr treatment with mLCN2, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.

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Recombinant mouse LCN2 (mLCN2) Gln21-Asn200 (Accession #P11672) was expressed in human 293 cells at Cell Signaling Technology.

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mLCN2 All lots are greater than 98% pure.

Molecular Formula:

Recombinant mLCN2 contains no "tags" and the nonglycosylated protein has a calculated MW of 20,874. DTT-reduced and non-reduced proteins migrate as 24 kDa polypeptides.

Bioactivity:

The bioactivity of mLCN2 was determined in a BAF3 cell viability assay. The ED50 of each lot is between 0.5-4 µg/ml.

Endotoxin:

Less than 0.01 ng endotoxin/1 μg mLCN2.

Formulation:

With carrier: A 0.22 μm filtered solution of 0.33 mg/ml mLCN2 in 20 mM Tris, pH 8.0 containing 150 mM NaCl and 20 μg BSA per 1 μg mLCN2. Carrier free: A 0.22 μm filtered solution of 0.33 mg/ml mLCN2 in 20 mM Tris, pH 8.0 containing 150 mM NaCl.

Storage:

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Lipocalin-2, a member of the lipocalin family of proteins, was originally identified as a gelatinase-associated component of neutrophil secretory granules (1). Lipocalin-2 is involved in innate immunity, iron homeostasis, and apoptosis. Lipocalin-2 limits bacterial growth by binding to bacterial siderophores and sequestering iron (2-4). In mammalian cells, iron-loaded lipocalin-2 binds to its receptor, 24p3R, and is internalized, thereby releasing iron and increasing the intracellular iron concentration (5). Conversely, iron-free lipocalin-2 promotes apoptosis (5). Lipocalin-2 is also expressed in adipose tissue and promotes insulin resistance in cultured mouse adipocytes (6).

  1. Kjeldsen, L. et al. (1994) Blood 83, 799-807.
  2. Flo, T.H. et al. (2004) Nature 432, 917-21.
  3. Goetz, D.H. et al. (2002) Mol Cell 10, 1033-43.
  4. Bachman, M.A. et al. (2009) PLoS Pathog 5, e1000622.
  5. Devireddy, L.R. et al. (2005) Cell 123, 1293-305.
  6. Yan, Q.W. et al. (2007) Diabetes 56, 2533-40.
Entrez-Gene Id
16819
Swiss-Prot Acc.
P11672
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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