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W           H M R Mk
W           H M R Mk
33 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

SF2/ASF Antibody recognizes endogenous levels of total SF2/ASF protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala171 of human SF2/ASF protein. Antibodies are purified by protein A and peptide affinity chromatography.

SF2/ASF is a member of the Ser-Arg-rich (SR) protein family of highly conserved nuclear phosphoproteins involved in pre-mRNA splicing (1). Besides its role in nuclear pre-mRNA splicing, SF2/ASF has been shown to shuttle between the nucleus and cytoplasm, suggesting additional roles in mRNA transport and cytoplasmic events (2). SF2/ASF associates with translating ribosomes and stimulates translation (3). It also activates translation initiation by suppressing the activity of 4E-BP1, which is mediated by SF2/ASF association with mTOR and the phosphatase PP2A (4). More recent studies have demonstrated a role for SF2/ASF in microRNA processing (5).

1.  Screaton, G.R. et al. (1995) EMBO J 14, 4336-49.

2.  Cáceres, J.F. et al. (1998) Genes Dev 12, 55-66.

3.  Sanford, J.R. et al. (2004) Genes Dev 18, 755-68.

4.  Michlewski, G. et al. (2008) Mol Cell 30, 179-89.

5.  Wu, H. et al. (2010) Mol Cell 38, 67-77.

Entrez-Gene Id 6426
Swiss-Prot Acc. Q07955

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SF2/ASF Antibody