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Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb (PE Conjugate) #8497
This product is discontinued
Gallery: Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb (PE Conjugate) #8497
Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of cyclin D1 only when phosphorylated at Thr286. This antibody does not cross-react with other cyclin D family members.Species predicted to react based on 100% sequence homology: Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of cyclin D1 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300.
Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc.