Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Important Shipping Information

Due to a water main break, our U.S. warehouse is closed for the remainder of the day, Thursday, July 28. Orders placed today will ship tomorrow, Friday, July 29. We apologize for the inconvenience.

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

We recommend the following alternatives

W IP   IF     H M R
REACTIVITY SENSITIVITY MW (kDa) SOURCE
96 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Dab2 Antibody recognizes endogenous levels of total Dab2 protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro222 of human Dab2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Disabled homologue 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4) and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is down-regulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18).


1.  Zhou J et al. (2003) J Biol Chem 278, 6936–41

2.  Hocevar, B.A. et al. (2001) EMBO J 20, 2789-801.

3.  Hocevar, B.A. et al. (2005) J Biol Chem 280, 25920-7.

4.  Hocevar, B.A. et al. (2003) EMBO J 22, 3084-94.

5.  Xu, X.X. et al. (1998) Oncogene 16, 1561-9.

6.  Mishra, S.K. et al. (2002) EMBO J 21, 4915-26.

7.  Morris, S.M. and Cooper, J.A. (2001) Traffic 2, 111-23.

8.  Keyel, P.A. et al. (2006) Mol Biol Cell 17, 4300-17.

9.  Maurer, M.E. and Cooper, J.A. (2006) J Cell Sci 119, 4235-46.

10.  Maurer, M.E. and Cooper, J.A. (2005) J Cell Sci 118, 5345-55.

11.  Morris, S.M. et al. (2002) Traffic 3, 331-41.

12.  Hasson, T. (2003) J Cell Sci 116, 3453-61.

13.  Huang, C.L. et al. (2006) J Cell Sci 119, 4420-30.

14.  Teckchandani, A. et al. (2009) J Cell Biol 186, 99-111.

15.  Mok, S.C. et al. (1998) Oncogene 16, 2381-7.

16.  Anupam, K. et al. (2006) World J Gastroenterol 12, 6041-5.

17.  Bagadi, S.A. et al. (2007) Breast Cancer Res Treat 104, 277-86.

18.  Chetrit, D. et al. (2011) J Biol Chem 286, 5392-403.


Entrez-Gene Id 1601
Swiss-Prot Acc. P98082


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

8582
Dab2 Antibody