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8629
SignalKine™ Human IL-2 Chemiluminescent Sandwich ELISA Kit

SignalKine™ Human IL-2 Chemiluminescent Sandwich ELISA Kit #8629

This product is discontinued

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Figure 1. PBMCs were initially stimulated with immobilized anti-CD3 (20 mg/well), soluble anti-CD28 antibody (1 μg/ml), recombinant hIL-4 #8919 (1 ng/ml), and recombinant hIL-2 #8907 (2 ng/ml) for a total of 5 d. The cells were then washed and either untreated (-) or treated (+) with TPA #4174 (10 ng/ml) and ionomycin (1 μg/ml). Cell culture media supernatants were removed after 4 and 24 hr and hIL-2 levels were quantified using SignalKine™ Human IL-2 Chemiluminescent Sandwich ELISA Kit.

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Typical Data. Represented above are hIL-2 Standard Curves, one diluted in SignalKine™ Sample Diluent S01, a second diluted in SignalKine™ Sample Diluent S03, and a third in cell culture media (RPMI + 10% FBS). Although these are typical of the standard curves that will be generated using this kit, a new set of standards should be run for each new experiment.

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Product Includes Volume Solution Color
hIL-2 Mouse mAb Coated Microwells (Chemiluminescent) 96 tests
Human IL-2 Standard (Lyophilized) 1 vial
SignalKine™ Assay Diluent A01 1.5 ml Colorless
SignalKine™ Sample Diluent S01 25 ml Brown
SignalKine™ Sample Diluent S03 25 ml Brown
hIL-2 Detection Rabbit mAb (Biotinylated)(Lyophilized) 100X 1 vial
Detection Antibody Diluent 6 ml Green
HRP-linked Streptavidin (Lyophilized) 100X 1 vial
HRP Diluent 6 ml Red
ELISA Wash Buffer (20X) 25 ml Colorless
Luminol/Enhancer Solution 3 ml Colorless
Stable Peroxide Buffer 3 ml Colorless
Sealing Tape 2 ea

SignalKine™ Human IL-2 Chemiluminescent Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human IL-2 (hIL-2) in multiple matrices. Unknown samples being tested for hIL-2 and hIL-2 standards are added to low volume microwells, where the hIL-2 is captured by the coated hIL-2 Mouse mAb. Following a washing step, a biotinylated hIL-2 Detection Rabbit mAb is added to detect the captured hIL-2. HRP-linked Streptavidin is then used for detection of the biotinylated hIL-2 Detection Rabbit mAb. A chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU) is proportional to the quantity of human hIL-2 in the sample. SignalKine™ Human IL-2 Chemiluminescent Sandwich ELISA Kit detects hIL-2 in multiple matrices that can be quantified by generating a standard curve with the recombinant hIL-2 protein standard provided. The hIL-2 standard range is from 31.2 to 2000 pg/ml. Samples containing higher levels of hIL-2 can be diluted to fit into the standards range.

Species Reactivity:

Human

Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).

  1. Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79.
  2. Gaffen, S.L. and Liu, K.D. (2004) Cytokine 28, 109-23.
  3. Fehérvari, Z. et al. (2006) Trends Immunol 27, 109-11.
  4. Antony, P.A. et al. (2006) J Immunol 176, 5255-66.
  5. Jaleco, S. et al. (2003) J Immunol 171, 61-8.
Entrez-Gene Id
3558
Swiss-Prot Acc.
P60568
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.