The Cellular Localization Alexa Fluor® 647 Conjugated Antibody Sampler Kit provides an economical means for identification of cellular organelles without the need for a fluorescent secondary antibody. This kit includes enough primary antibody to perform at least 20 IF-IC tests per antibody.
β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total β-actin protein. This antibody may cross-react with the γ-actin (cytoplasmic isoform), but it does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms. COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total COX IV protein. PDI (C81H6) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total PDI protein. β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total β-tubulin protein and does not cross-react with recombinant α-tubulin.
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-actin protein, residues surrounding Lys29 of human COX IV protein, the sequence of human PDI protein, or the amino terminus of human β-tubulin protein.
Knowledge of the subcellular location of a protein may reveal the potential role it plays in the cell. The subcellular location of a protein of interest may be confirmed by colocalization with one of the organelle-specific antibodies in this kit.
β-Actin and β-tubulin are ubiquitous eukaryotic proteins that form the major component of the cellular cytoskeleton (1,2). Cytochrome c oxidase (COX) IV is part of the hetero-oligomeric enzyme found in the inner mitochondrial membrane (3). Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of disulfide bonds in the endoplasmic reticulum (4).
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