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97 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Importin β1 Antibody detects endogeneous levels of total importin β1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro307 of human importin β1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Importins belong to the karyopherin family of nuclear transport proteins (1) and are divided into two subgroups: importin α and importin β. Importins mainly function in nuclear protein import and export (2,3). Importin β1 (also known as karyopherin β1, Kpnβ1, Kpnb1, or p97) plays a key role in the nuclear import process (1-3). Nuclear import via importin β1 association with adaptor importin α (also known as karyopherin α, or Kpnα) is an essential component of the classical nuclear localization signal (NLS) pathway (4). Importin α directly recognizes the NLS present in the cargo target, prompting complex formation with importin β1. The cargo:importin α:importin β1 complex is transported across the nuclear pore complex (NPC) into the nucleus, where it is dissociated by the binding of RanGTP (1-4). Nuclear import directly via importin β1 can also occur by importin β1 recognition of the cargo protein, bypassing importin α involvement. In both cases, the importin β1/target protein interaction is mediated through the binding of importin β1 HEAT repeats with the target protein sequences (either the cargo protein itself or importin α) (5).

1.  Chook, Y.M. and Blobel, G. (2001) Curr Opin Struct Biol 11, 703-15.

2.  Pemberton, L.F. and Paschal, B.M. (2005) Traffic 6, 187-98.

3.  Moroianu, J. (1998) J Cell Biochem 70, 231-9.

4.  Lange, A. et al. (2007) J Biol Chem 282, 5101-5.

5.  Marfori, M. et al. (2011) Biochim Biophys Acta 1813, 1562-77.

Entrez-Gene Id 3837
Swiss-Prot Acc. Q14974

For Research Use Only. Not For Use In Diagnostic Procedures.
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Importin β1 Antibody