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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Annexin A1 (D16A10) Rabbit mAb recognizes endogenous levels of total annexin A1 protein. This antibody does not cross-react with other members of the annexin family.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val236 of human annexin A1 protein.

The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology (1). Annexin-1 (ANXA1) is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis (2-4). Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity (5,6). Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis and may be required for a late stage in inward vesiculation (7). Phosphorylation by PKC, EGFR, and Chak1 results in inhibition of annexin A1 function (8-10). Annexin A1 has also been identified as one of the 'eat-me' signals on apoptotic cells that are to be recognized and ingested by phagocytes (11). Annexin A1, as an endogenous anti-inflammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development (12-14).

1.  Raynal, P. and Pollard, H.B. (1994) Biochim Biophys Acta 1197, 63-93.

2.  Blackwell, G.J. et al. (1980) Nature 287, 147-9.

3.  Rothhut, B. et al. (1983) Biochem Biophys Res Commun 117, 878-84.

4.  Hirata, F. et al. (1981) Proc Natl Acad Sci USA 78, 3190-4.

5.  Kim, K.M. et al. (1994) FEBS Lett 343, 251-5.

6.  Kim, S.W. et al. (2001) J Biol Chem 276, 15712-9.

7.  White, I.J. et al. (2006) EMBO J 25, 1-12.

8.  Varticovski, L. et al. (1988) Biochemistry 27, 3682-90.

9.  Dorovkov, M.V. and Ryazanov, A.G. (2004) J Biol Chem 279, 50643-6.

10.  Wang, W. and Creutz, C.E. (1994) Biochemistry 33, 275-82.

11.  Arur, S. et al. (2003) Dev Cell 4, 587-98.

12.  Perretti, M. and Gavins, F.N. (2003) News Physiol Sci 18, 60-4.

13.  Parente, L. and Solito, E. (2004) Inflamm Res 53, 125-32.

14.  Lim, L.H. and Pervaiz, S. (2007) FASEB J 21, 968-75.

Entrez-Gene Id 301
Swiss-Prot Acc. P04083

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The Annexin V-FITC conjugate is a product manufactured for Cell Signaling Technology, Inc. by TAU Technologies BV.

Annexin A1 (D16A10) Rabbit mAb